Preliminary Course Date: April 5-8, 2011, Breda (NL) Last update: 15-07-2010
This course is organised in collaboration with Dr. J.T. den Dunnen, Leiden Genome Technology Center, Human Genetics, LUMC, Leiden
Aim
of the course:
DNA sequencing technologies are currently developing at an incredible
speed and new applications are presented monthly, incl. DNA/RNA profiling,
ChIP-seq, re-sequencing, de novo genome sequencing and metagenomics. The
aim of this course is to introduce next generation sequencing technologies,
highlight different applications of these new technologies, and show ways
to analyse data.
Participants:
This
course has been developed for technicians and PhD students who are interested
in, planning, or already working with next-generation sequencing. We welcome
researchers from both the genomics and bioinformatics fields. Currently
available technologies, applications, as well as hardware and software solutions
will be presented and discussed.
Course structure:
We offer a three-day short-course consisting of a series of lectures on
the technologies and applications of next generation sequencing and "hands-on"
data analysis practicals. Furthermore we offer an additional course day
for those partcipants who would like to get more skills in Linux based GAPSS
data analysis pipelines. All participants will receive a practical manual
containing all used protocols.
Preliminary Topics included in the course:
3-day
Course 2542
Next generation sequencing technologies and applications
Tuesday February 9, 2010
Lectures:
Roche / 454 technology platform
ABI / SOLID technology platform
Illumina / Solexa technology platform
ChIP-seq
De novo sequencing
miRNA profiling
Computer practicals:
Genome browser views: ChIP-seq, De novo sequencing, and miRNA profiling
Wednesday February 10, 2010
Lectures:
Hyb capture and Long range PCR and pooling
Simultaneous mutation and copy number variation (CNV) detection by multiplex
PCR Based GS-FLX sequencing
Paired end mapping
Re-sequencing and SNP discovery
Introduction to CLCbio data analysis
Computer Practicals:
Genome browser views Long range PCR and pooling, Hyb capture, and Paired
end mapping
Windows practical: CLCbio Workbench
Thursday June 11, 2010
Lectures:
Metagenomics
Introduction to Nextgene data analysis
RNA expression profiling
Future developments “2013”
Computer practical:
Windows practical: Nextgene data analysis
EXTRA DAY Course 2542b
Data analysis pipelines
Friday February 12, 2010
Lectures:
Intoduction to Linux
Alignment and assembly
Publicly available software
Introduction to pipelines and GAPSS
Computer practicals:
Linux practical, the basics
Alignment and assembly
GAPSS and UCSC viewing
Avans Hogeschool reserves the right to substitute speakers, and change the theoretical and practical program, in order to cover most current and future new sequening technologies applications.
Course staff:
Course
leaders:
Dr.
M.C.M. Verschuren, Avans Hogeschool, Research group Analysis Techniques
in Life Science, Breda, NL
Course co-ordinator:
Dr. J.T. den Dunnen, Leiden Genome Technology Center, Human Genetics,
LUMC, Leiden.
Accreditation:
All
participants will receive a certificate of attendance
General
Information:
Code: 2542
(3-day course)
Registration fee: € 800,- The fee includes course materials
and lunch
Code: 2542b
(1 day additional course)
Registration fee: € 300,- The fee includes course materials
and lunch
Language:
English
Location : Avans
Hogeschool, Lovensdijkstraat 61, Breda, The Netherlands route
description
Sponsors:
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